Effect of intravenous low-intensity laser irradiation of the blood on clinical and laboratory parameters of hepatocellular insufficiency.

Patients with hepatocellular insufficiency received a course of intravenous laser irradiation of the blood. After the treatment, a positive dynamics of clinical and biochemical indices of the major hepatic syndromes was observed: alleviation of the major clinical symptoms and significant positive changes in biochemical parameters (AST, ALT, bilirubin, alkaline phosphatase, lactate dehydrogenase, and total cholesterol).

[The role of cardiovascular diseases among the reasons for the transfer ahead of schedule of specialists working in the tropics]. / Rol' serdechno-sosudistykh zabolevanii sredi prichin dosrochnogo otkomandirovaniia spetsialistov, rabotaiushchikh v tropikakh.

The authors have considered the causes of inability to continue working in tropic climate because of health deterioration for 359 specialists and 19 lethal cases. The main cause of inability to work in tropical climate and primary cause of death was cardiovascular disease. One-third of the cases were due to exacerbation of chronic diseases. The exacerbations occurred more frequently in the most intensive phases of adaptation. Measures which should be taken to prevent breakdown of health in subjects working in tropical conditions are recommended.

[The stress-modulating effects of laser therapy in IHD patients]. / Stress-moduliruiushchie éffekty lazeroterapii u bol'nykh IBS.

Dependence of the response to helium-neon laser therapy (LT) on initial level and changes of blood plasma hydrocortisone (HC), testosterone (T), diene conjugates (DC) concentration, general antioxidant activity (AOA) of blood plasma, TxB and 6-keto-PGF were estimated in 147 patients with ischemic heart disease and stable angina. The response to LT was observed in patients with high levels of HC, T, AOA and 6-keto-PGF1 alpha. A significant increase of DC was noted in these patients too. The minimal level of HC. T and AOA was recorded in patients with negative clinical effect. The treatment only aggravated HC, T and AOA deficiency and raised DC and TxB level. The findings suggest that the effect of LT is based on modulation of nonspecific adaptive mechanisms.

[Effect of irradiation of blood with a helium-neon laser on aggregation of neutrophilic granulocytes of patients with ischemic heart disease]. / Vliianie oblucheniia krovi gelii-neonovym lazerom na agregatsiiu neitrofil'nykh granulotsitov bol'nykh ishemicheskoi bolezn'iu serdtsa.

Blood samples from 30 patients with myocardial ischemia and 25 healthy donors were irradiated with He-Ne laser. ATP release was measured by the luciferin-luciferase test system on a PICA lumo-aggregometer (Chrono-Log Corporation, USA). There was no correlation between lectin-induced ATP release and cell aggregation. Neutrophils obtained from donors and patients differed in the number of lectin receptors, including mannose and sialic acids on the cellular surface. Following laser irradiation, WGA- and SBA-induced aggregation was decreased, while Con-A-induced aggregation was increased.

[Metabolism of several blood prostaglandins in patients with angina pectoris during helium-neon laser therapy]. / Metabolizm nekotorykh prostaglandinov krovi u bol'nykh so stenokardiei na fone gelii-neonovoi lazernoi terapii.

The paper shows the high therapeutical efficiency of combined He-Ne laser therapy in patients with coronary heart disease. Its effect on the thromboxane-prostacyclin system is one of the mechanisms of laser impact. The combined He-Ne laser therapy promotes normalization of the level of thromboxane B2 and its ratio to the metabolite prostacyclin.

[Lectin-induced chemiluminescence of peripheral blood neutrophils in patients with ischemic heart disease before and after blood irradiation with helium-neon laser]. / Lektinindutsirovannaia khemiliuminestsentsiia neitrofil'nykhgranu- lotsitov perifericheskoi krovi bol'nykh ishemicheskoi bolezn'iu serdtsa do i posle oblucheniia krovi gelii-neonovym lazerom.

The blood taken from 35 patients with coronary heart disease and 30 healthy donors was irradiated with He-Ne laser, which resulted in a decrease in its count of segmented neutrophilic granulocytes. Lectins bound to various carbohydrate determinants onto the neutrophil surface were shown to affect changes occurring after luminol-depended chemiluminescence irradiation in patients and healthy persons in different ways. The patients' neutrophils contained lower levels of radiommunologically detectable leukotriene B4. Thromboxane B2 levels also dropped following the irradiation. The laser irradiation induced elimination of some less resistant cells from blood flow, i.e. "rejuvenation" of a cell population of neutrophilic granulocytes. The remaining cells differed in the composition and reactivity of surface lectin receptors and in the content of biologically active substances, which is likely to play the key role in the mechanism responsible for the therapeutical effect of He-Ne laser.

[Natural anti-PGF2alpha and anti-PGE2 antibodies in ischemic heart disease and hypertension]. / Estestvennye antitela k prostaglandinam F2alpha i E2 pri ishemicheskoi bolezni serdtsa i gipertonicheskoi bolezni.

The important role played by prostaglandins in the pathogenesis of coronary disease and essential hypertension is well known. The authors have attempted to reveal a relationship between blood levels of natural autoantibodies to PGF2 alpha and PGE2 and specific features of coronary disease and essential hypertension course and complications. A total of 87 subjects were examined, 23 of these--normal controls, 25 patients with myocardial infarction, 22 with angina pectoris, and 17 with essential hypertension. Solid-phase enzyme immunoassay was employed to detect anti-PGF2 alpha and anti-PGE2 antibodies. These antibodies were found in normal subjects, coronary patients and hypertensives. Blood levels of these antibodies correlated with some complications of coronary disease and essential hypertension. These results permit a hypothesis that the pathogenetic physiologic role of the detected natural antibodies to prostaglandins consists in their defense homeostatic function.

[Laser treatment of patients with chronic glomerulonephritis]. / Lazernaia terapiia bol'nykh khronicheskim glomerulonephritis.

Helium-neon laser therapy of patients suffering from mixed and nephrotic glomerulonephritis demonstrated hypotensive, diuretic and fibrinolytic activity boosting clinical effects. The use of the new treatment method seems to be justified, since all the patients given laser therapy manifested pronounced resistance to the pathogenetic therapy carried out previously (glucocorticoids, cytostatics, hypotensive and diuretic drugs). The presence of diverse effects and lack of complications suggest a broader-scale use of laser therapy in nephrology. At present the authors are analyzing the ++patho-chemical bases of the therapeutic efficacy of laser therapy of patients suffering from chronic glomerulonephritis. The results will be reported in the next paper.

[Incidence of acute myocardial infarction among Soviet specialists after long-term work in the tropics]. / Zabolevaemost' ostrym infarktom miokarda Sovetskikh spetsialistov, dlitel'no rabotaiushchikh v tropikakh.

The paper presents the results of epidemiological survey of acute myocardial infarction morbidity rates among the Soviet specialists who worked in the Republic of Cuba. A total of 54,765 individuals aged 20-59 years were examined within 10 years. There were 48 cases of acute myocardial infarction (AMI). The morbidity rate in males and females was 1.44 and 0.08 per 1,000, respectively. The morbidity rate was 22.9%; death rate in males and females was 0.28 and 0.08 per 1,000, respectively. The incidence of major risk factors in AMI patients and the relation of the morbidity rates of the specialists to the specific features of their adaptation to an emergency of the tropics were considered. Some trends of practical measures were defined to reduce AMI morbidity rates in specialists moving to the tropics to work.

[The regulatory role of natural antibodies and prostaglandin F2 alpha in the course of a myocardial infarct]. / Reguliatornaia rol' estestvennykh antitel i prostaglandinov F2 al'fa v techenii infarkta miokarda.

Concentration of PGF2 alpha were correlated to those of antiprostaglandin antibodies IgM and IgG to this mediator in donors and myocardial infarction patients. In donors correlation between PGF2 alpha concentration and IgG antibody levels is positive. In uneventful myocardial infarction relevant correlation partially recovered at the expense of IgM antibodies. This is not true for complicated myocardial infarction.

[Clinical and prognostic value of monitoring the acute phase protein levels in patients with myocardial infarction]. / KLiniko-prognosticheskaia znachimost' monitorirovaniia belkov ostroi fazy u bol'nykh infarktom miokarda.

The value of evaluating the protein levels at the acute stage was examined to define the clinical course and prognosis of myocardial infarction. The time course of hospital myocardial infarction was studied in 117 patients. The Manchini radial immunodiffusion with reference monospecific antisera was employed to determine individual proteins during the acute stage (orosomucoid, C-reactive protein, alpha 1-antitrypsin, C3 complement, ceruloplasmin). The level of circulating immune complexes was measured by polyethylene glycol precipitation. There was a close correlation between the circulating immune complex levels and some acute phase parameters, suggesting the relationship between immune and inflammatory processes. The changes in the magnitude of some acute phase parameters and circulating immune complexes were found to be significant in predicting the development of complications and the fatal outcome of the disease.

[Comparative analysis of thrombin and anti-thrombin antibody levels in donors and myocardial infarction patients]. / Sravnitel'nyi analiz soderzhaniia trombina i antitel k nemu u bol'nykh infarktom miudarda.

Thrombin and natural antithrombin antibody content was comparatively studied in donors and myocardial infarction patients. It was shown that the level of thrombin and antithrombin antibody was stable in the group of clinically normal subjects. In patients with myocardial infarction an increased thrombin level attended by a decrease in the level of natural antithrombin antibody was recorded. The presence of linear correlation between the levels of thrombin and antithrombin antibody, as well as impossibility of the diagnosis of disorders in the blood coagulation system using one of the parameters studied have permitted introduction of an additional diagnostic parameter--"antithrombin index."

[Changes in the superoxide dismutase activity during stimulation of polymorphonuclear leukocytes of peripheral blood]. / Izmenenie aktivnosti superoksiddismutazy v protsesse stimuliatsii polimorfnoiadernykh leikotsitov perifericheskoi krovi.

The functional activity of the peripheral blood polymorphonuclear leukocytes (PML) was investigated by using the method of latex-stimulated luminol-dependent chemiluminescence (CL). The CL-intensity of PML taken from patients with acute myocardial infarction (MI) was found to be 20 times higher than that of normal individuals (NI). The change in activity of endogenous antioxidative enzyme systems may account for alteration of PML CL-parameters. It was established that the initial superoxide dismutase (SOD) activity of unstimulated PML from patients with MI exceeds that of NI, and that rapid increase in intercellular SOD activity (within 30 sec.) occurs in the process of PML stimulation. It was suggested that the change of SOD activity during PML stimulation was the result in the enzyme partial proteolysis in the cells. The positive correlation between initial level of SOD activity and CL-intensity of PML was observed. The investigation of the above parameters in MI dynamic showed a gradual normalization of PML CL-response and insignificant decrease in intracellular SOD activity in case of a favourable cause of the disease. Increased SOD activity in PMLs may be one of the factors contributing to a decrease in PML functional activity in the disease dynamic.

[Combined helium-neon laser therapy in patients with ischemic heart disease]. / Kombinirovannaia gelii-neon-lazernaia terapiia u bol'nykh ishemicheskoi bolezn'iu serdtsa.

The paper describes the combined helium-neon-laser (HNL) therapy (intravenous and topical) developed by the authors to treat patients with coronary heart disease. A high efficacy of this therapy mode was demonstrated in patients over 70 years of age with Functional Classes III-IV angina refractory to antianginal agents. The mechanisms responsible for therapeutic efficiency of laser irradiation were studied at the membraneous and cellular levels. There is evidence that the combined HNL-therapy had advantages over topical HNL exposure in terms of higher clinical efficiency and patterns of abnormal chemical changes.

[Helium-neon laser therapy in the combined treatment of unstable stenocardia]. / Gelii-neonovaia lazernaia terapiia v kompleksnom lechenii nestabil'noi stenokardii.

He-Ne laser therapy included in complex of therapeutic methods for patients with unstable angina pectoris is a highly effective treatment modality; it helps essentially reduce the risk of acute myocardial infarction in these patients. Clinical efficacy of laser therapy is confirmed by its favorable action on hemostasis plasma factors, consisting in reduction of fibrinogen level, normalization of antithrombin-III (AT-III), decrease of the level of soluble fibrinomonomer complexes, this indicating a lowering of the blood coagulation potential. Absence of significant changes in plasminogen level may be an indicator of the nonenzymic route of fibrinogen system activation. Sessions of intravenous laser therapy should be administered 2-3 times a week to unstable angina pectoris patients with low AT-III levels, whereas for patients with initially high or normal AT-III levels combined laser therapy is advisable (4-5 daily invasive procedures and 6-8 skin surface ones on the Zakharyin-Head's zones). Measurements of endogenic anticoagulants is an effective means for monitoring laser therapy in this patient population.

[Thrombin and anti-thrombin antibody levels in the blood of patients with acute myocardial infarction]. / Dinamika soderzhaniia trombina i antitel k nemu v krovi bol'nykh ostrym infarktom miokarda.

Simultaneous levels of thrombin and its natural antibodies were comparatively analyzed in donors and patients with myocardial infarction. Thrombin and its antibody levels were demonstrated to remain unchanged in the healthy subjects. With myocardial infarction development, there was an increase in thrombin concentrations and a decrease in natural antithrombin antibodies at the same time. A linear correlation was established between the levels of thrombin and its antibodies and failure in diagnosing coagulative disorders by one of the parameters studied was also ascertained, which made it possible to introduce a new diagnostic criterion, namely an "antithrombin index" that enabled the groups of donors and patients to be differentiated.